Resuscitate early with plasma and platelets or balance blood products gradually

To resuscitate or not to resuscitate.

You pull up on scene of a rollover to transport a trauma victim or you are standing in the ED waiting for the ambulance to bring in the trauma victim. One of the first things you might be thinking is, “I need two large bore IVs with warmed fluids running wide open!” However, current evidence-based practice is trending to very judicious use of IV fluids. Where it was once thought that more fluid...

Hyperimmunes Plasma Procurement and Products

Isolation and characterization of plasma membranes from human blood platelets.

Human platelets were subjected to hypoto?ic lysis after being loaded intracellularly with glycerol (4.3 M) by centrifugation in a glycerol gradient under controlled conditions. A homogeneous membrane fraction, free of intracellular organelles, was isolated after a single density step centrifugation, while continuous sucrose density gradient centrifugation gave two subfractions with densities of...

Stability of catecholamines in whole blood, plasma, and platelets.

Checking catecholamine stability in whole blood, plasma, and platelets, we found that specimens stored at room temperature or frozen for periods ranging from 1.5 h to three weeks show no significant difference in measured catecholamine concentration. The implications of these findings are discussed.

Rate of Glucose Utilization by Blood Cells in Serum and Plasma Specimens With or Without Using Preservative

Objective: This study aims to estimate and compare the time-course change in blood glucose levels by blood cells in serum, and plasma with or without preservatives, which may reflect the rate of glucose utilization by blood cells. Method: This laboratory-based cross-sectional study was carried out using a blood specimen of 28 participants among which 14 were diagnosed with diabetes and 14 were...